Affinity purification of monoclonal antibodies, using a bifunctional oligosaccharide hapten
โ Scribed by Srinivas Uppugunduri; Jamal Dakour; Elisabeth Kallin; Thomas Norberg; David Zopf; Arne Lundblad
- Publisher
- Springer US
- Year
- 1991
- Tongue
- English
- Weight
- 844 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1573-4986
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๐ SIMILAR VOLUMES
An affinity purification technique has been developed using mild elution conditions for the isolation of humanized murine and murine \(\mathrm{IgG}_{1}\). This technique is based on the innate affinity of \(\mathrm{IgG}_{1}\) for metal and utilizes immobilized metal-affinity chromatography. Antibody
## Abstract We evaluated the potential for polyelectrolyte induced precipitation of antibodies to replace traditional chromatography purification. We investigated the impact of solution pH, solution ionic strength and polyelectrolyte molecular weight on the degree of precipitation using the anionic