Affinity purification of antibodies using immobilized FB domain of protein A
β Scribed by Beka Solomon; Osnat Raviv; Efrat Leibman; Gideon Fleminger
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 484 KB
- Volume
- 597
- Category
- Article
- ISSN
- 1873-3778
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Affinity systems based on specific molecular recognition are valuable tools for detection, purification and immobilization of recombinant proteins. Here, novel multipartite affinity fusion vectors were assembled and investigated to allow flexible binding and elution conditions. The rationale for the
An affinity purification technique has been developed using mild elution conditions for the isolation of humanized murine and murine \(\mathrm{IgG}_{1}\). This technique is based on the innate affinity of \(\mathrm{IgG}_{1}\) for metal and utilizes immobilized metal-affinity chromatography. Antibody