𝔖 Bobbio Scriptorium
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Affinity purification of elongation factors tu and Ts

✍ Scribed by Gary R. Jacobson; Jürg P. Rosenbusch

Book ID
115907453
Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
387 KB
Volume
79
Category
Article
ISSN
0014-5793

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📜 SIMILAR VOLUMES

Bulk preparation and crystallization of
Bulk preparation and crystallization of the Escherichia coli elongation factor Tu-Ts complex
✍ Marilyn Yoder; Clare Tores; Robin Corelli; Frances Jurnak 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 426 KB

A simple procedure for the preparation of lo-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by d