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The reactions of the sulfhydryl groups on the elongation factors Tu and Ts

โœ Scribed by D.L. Miller; J. Hachmann; H. Weissbach

Book ID
115701139
Publisher
Elsevier Science
Year
1971
Tongue
English
Weight
626 KB
Volume
144
Category
Article
ISSN
0003-9861

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Bulk preparation and crystallization of
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โœ Marilyn Yoder; Clare Tores; Robin Corelli; Frances Jurnak ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 426 KB

A simple procedure for the preparation of lo-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by d