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Affinity purification of dextransucrase from Streptococcus sanguis ATCC 10558

โœ Scribed by Susan F. Kobs; David W. Husman; Kevin M. Cawthern; Robert M. Mayer


Book ID
107725914
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
614 KB
Volume
203
Category
Article
ISSN
0008-6215

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Photolabeling of dextransucrase from Str
โœ Susan F. Kobs; Robert M. Mayer ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 567 KB

Dextransucrase from Streptococcus sanguis ATCC 10558 was photolabeled using p-azidophenyl alpha-D-glucopyranoside with an apparent rate constant of inactivation of 1.40 min-1. The dissociation constant for this compound, which acts as an acceptor molecule in the enzymatic reaction, is 90 microM. App