✦ LIBER ✦
Photolabeling of dextransucrase from Streptococcus sanguis with p-azidophenyl α-d-glucopyranoside
✍ Scribed by Susan F. Kobs; Robert M. Mayer
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 567 KB
- Volume
- 211
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Dextransucrase from Streptococcus sanguis ATCC 10558 was photolabeled using p-azidophenyl alpha-D-glucopyranoside with an apparent rate constant of inactivation of 1.40 min-1. The dissociation constant for this compound, which acts as an acceptor molecule in the enzymatic reaction, is 90 microM. Apparently two acceptor binding sites exist on dextransucrase as shown by (i.) photolabeling the enzyme with p-azidophenyl-alpha-D-[5,6-3H]glucopyranoside and (ii.) fluorescence titration experiments.