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Affinity mode of capillary isoelectric focusing for the characterization of the biotin-binding protein actinavidin

✍ Scribed by Dr. Vadim M. Okun


Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
595 KB
Volume
19
Category
Article
ISSN
0173-0835

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Competitive binding assays for high-affi
✍ Richard W. Schreiber Jr.; Michael A. Letavic; Timothy J. McGahan; Harold B. Whit πŸ“‚ Article πŸ“… 1991 πŸ› Elsevier Science 🌐 English βš– 731 KB

Endogenous ligands complicate radioligand-binding assays of high-affinity binding proteins by obscuring binding sites or by diluting the labeled ligand. We have developed a mathematical model for such systems where radioligand and endogenous ligand are structurally identical. Data which relate radio