Affinity maturation of anti-hapten antibodies in a single mouse analyzed by two-dimensional affinity electrophoresis
β Scribed by Prof. Kazuyuki Nakamura; Yusuke Mimura; Tatehiko Tanaka; Yoshihisa Fujikura; Kazusuke Takeo
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 318 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0173-0835
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The molecular heterogeneity of rabbit anti-hapten antibodies has been investigated by two-dimensional affinity electrophoresis (2D-AEP). Anti-dansyl and antiarsanilic diazo-antibodies were separated into several hundred IgG spots as in the case of anti-DNP antibodies. They were grouped into a number