Actin-binding proteins in mouse C2 myoblasts and myotubes: A combination of affinity chromatography and two-dimensional gel electrophoresis

Two-dimensional gel electrophoresis of a

Two-dimensional gel electrophoresis of actin-binding proteins isolated by affinity chromatography from human skeletal muscle

✍ Dr. Joëlle V. F. Coumans; Ian Humphery-Smith; Cristobal G. dos Remedios 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 697 KB

In muscle cells actin exists as a mixture of monomeric (G-actin) and filamentous actin (F-actin) and ionic conditions strongly favor the formation of F-actin. The existence of unpolymerized actin depends, among other factors, on proteins that bind to G-actin, the so-called G-actin-binding proteins (