We have developed a reagentless optical biosensor for glutamine based on the Escherichia coli glutamine binding protein (GlnBP). Site-directed mutagenesis was performed to engineer single cysteine mutants which were covalently modified with environmentally sensitive sulfhydryl-reactive probes. The f
✦ LIBER ✦
Affinity immobilization of a genetically engineered bifunctional hybrid protein
✍ Scribed by François Baneyx; Christine Schmidt; George Georgiou
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 651 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0141-0229
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