Affinity Extraction of Dye- and Metal Ion-Binding Proteins in Polyvinylpyrrolidone-Based Aqueous Two-Phase System
β Scribed by Sheryl Fernandes; Hyung-Seog Kim; Rajni Hatti-Kaul
- Book ID
- 115645987
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 133 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1046-5928
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PEG has been activated using epoxy-oxirane, epichlorohydrin and periodate based reactions. The coupling to activated PEG of several protein ligands of different sizes was investigated. Glutathione, trypsin inhibitor, Protein A and anti-BSA have been bound to PEG and used to increase the selectivity
## Abstract The partition behaviour of Hg (II) was studied in an aqueous polyethylene glycol (PEG) β (NH4)2SO4 two-phase system as a function of halide, halide concentration, and pH. For a system prepared by mixing equal volumes of 40 % (w/w) PEG (1550) with 40 % (w/w) (NH4)2SO4, Hg(II) remains alm