✦ LIBER ✦

Affinity chromatography of urate oxidase on 8-aminoxanthine-bound Sepharose

✍ Scribed by Takafumi Watanabe; Tetsuya Suga

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 309 KB
Volume: 86
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90758-3

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✦ Synopsis

An agarose derivative that is an affinity adsorbent for chromatography of mate oxidase was prepared by coupling X-aminoxanthine with epoxy-activated Sepharose 4B. This affinity adsorbent was stable in 0.1 M borate buffer(pH 10.0) at 4°C since no decrease of effective ligand content in the gel was found for 5 months. Chromatography of crude urate oxidases prepared from bovine kidney, rat liver, and yeast on the affinity column resulted in 2-to 88-fold purifications.

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