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Affinity Chromatography of Insulin with a Heptapeptide Ligand Selected from Phage Display Library

โœ Scribed by Hai-Qing Yu; Xiao-Yan Dong; Yan Sun

Book ID
111788203
Publisher
Springer
Year
2004
Tongue
English
Weight
311 KB
Volume
60
Category
Article
ISSN
0009-5893

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โœ Kok Lian Ho; Khatijah Yusoff; Heng Fong Seow; Wen Siang Tan ๐Ÿ“‚ Article ๐Ÿ“… 2002 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 179 KB

M13 phages that display random disulfide constrained heptapeptides on their gpIII proteins were used to select for high affinity ligands to hepatitis B core antigen (HBcAg). Phages bearing the amino acid sequences C-WSFFSNI-C and C-WPFWGPW-C were isolated, and a binding assay in solution showed that