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Affinity chromatography of frog epidermis dopa-oxidase

✍ Scribed by J. L. Iborra; E. Cortes; A. Manjon; J. Ferragut; F. Llorca


Book ID
112971609
Publisher
Springer-Verlag
Year
1976
Tongue
English
Weight
440 KB
Volume
1
Category
Article
ISSN
0273-2289

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An agarose derivative that is an affinity adsorbent for chromatography of mate oxidase was prepared by coupling X-aminoxanthine with epoxy-activated Sepharose 4B. This affinity adsorbent was stable in 0.1 M borate buffer(pH 10.0) at 4Β°C since no decrease of effective ligand content in the gel was fo