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Affinity chromatography of enzymes on an AMP-analogue: Specific elution of dehydrogenases from a general ligand

✍ Scribed by Rolf Ohlsson; Peter Brodelius; Klaus Mosbach

Book ID
115902963
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
384 KB
Volume
25
Category
Article
ISSN
0014-5793

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πŸ“œ SIMILAR VOLUMES

Determination of dissociation constants
Determination of dissociation constants for binary dehydrogenase-coenzyme complexes by (Bio)affinity chromatography on an immobilized AMP-analogue
✍ Peter Brodelius; Klaus Mosbach πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 511 KB

Various alcohol and lactate dehydrogenases were adsorbed to an affinity column of immobilized W-(6-aminohexyl)-AMP and subsequently eluted with gradients of coenzymes or coenzyme fragments. A linear relation was observed between the eluting concentration of nucleotide and the reported dissociation c