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Separation of Isozymes of horse liver alcohol dehydrogenase and purification of the enzyme by affinity chromatography on an immobilized AMP-analogue

✍ Scribed by Lars Andersson; Hans Jörnvall; Åke Åkeson; Klaus Mosbach

Book ID
115733571
Publisher
Elsevier Science
Year
1974
Weight
445 KB
Volume
364
Category
Article
ISSN
0005-2744

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Determination of dissociation constants
Determination of dissociation constants for binary dehydrogenase-coenzyme complexes by (Bio)affinity chromatography on an immobilized AMP-analogue
✍ Peter Brodelius; Klaus Mosbach 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 511 KB

Various alcohol and lactate dehydrogenases were adsorbed to an affinity column of immobilized W-(6-aminohexyl)-AMP and subsequently eluted with gradients of coenzymes or coenzyme fragments. A linear relation was observed between the eluting concentration of nucleotide and the reported dissociation c