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Affinity chromatography by enzyme-substrate interaction. Purification of some rat liver glycosidases

โœ Scribed by Enrique Junowicz; Joseph Edward Paris

Book ID
115733159
Publisher
Elsevier Science
Year
1973
Weight
715 KB
Volume
321
Category
Article
ISSN
0005-2744

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Purification of pigeon liver malic enzym
Purification of pigeon liver malic enzyme by affinity chromatography
โœ Jaw-Tang Chang; Gu-Gang Chang ๐Ÿ“‚ Article ๐Ÿ“… 1982 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 336 KB

A simple and rapid method for the purification of malic enzyme (EC 1.1.1.40) from pigeon liver is described. Malic enzyme in the crude tissue extract was partially purified by heat treatment, ammonium sulfate fractionation, and DEAE-cellulose chromatography. Final purification was achieved by affini