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Affinity Chromatographic Selection of Carbonylated Proteins Followed by Identification of Oxidation Sites Using Tandem Mass Spectrometry

โœ Scribed by Mirzaei, Hamid; Regnier, Fred

Book ID
120063686
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
154 KB
Volume
77
Category
Article
ISSN
0003-2700

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An adduct with phenylhydrazine was formed with the purified pig kidney diamine oxidase and in parallel with the \(l\)-tyrosine decarboxylase from Streptococcus faecalis. The labeled enzymes were hydrolyzed by chemical hydrolysis and the adducts released by hydrolysis were isolated and identified fir