Advances in on-line monitoring and control of mammalian cell cultures: Supporting the PAT initiative
β Scribed by A.P. Teixeira; R. Oliveira; P.M. Alves; M.J.T. Carrondo
- Book ID
- 113494733
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 308 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0734-9750
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The use of on-line relative permittivity (Π) measurements for monitoring cultures of Sf-9 cells was evaluated in a batch culture and a batch infected with a baculovirus expressing β€-galactosidase. It was found that viable cell density and volume essentially accounted for all the variation in Π in bo
One of the requirements for enhanced productivity by the animal culture systems used in biotechnology is the direct assessment of the metabolic rate by on-line biosensors. Based on the fact that cell growth is associated with an enthalpy change, it is shown that the specific heat flow rate is stoich