𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Advances in animal cell recombinant protein production: GS-NS0 expression system

✍ Scribed by Louise M. Barnes; Catherine M. Bentley; Alan J. Dickson

Book ID
110259604
Publisher
Springer
Year
2000
Tongue
English
Weight
144 KB
Volume
32
Category
Article
ISSN
0920-9069

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Characterization of the stability of rec
Characterization of the stability of recombinant protein production in the GS-NS0 expression system
✍ Louise M. Barnes; Catherine M. Bentley; Alan J. Dickson 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 670 KB

## Abstract The GS‐NS0 system is an important mammalian expression system used largely within industry for the high‐level expression of recombinant proteins for therapeutic use. It is essential that the productivity of this system remains stable throughout culture expansion for the successful long‐

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## Abstract Presented is a novel antibody production platform based on the fed‐batch culture of recombinant, NS0‐derived cell lines. A standardized fed‐batch cell culture process was developed for five non‐GS NS0 cell lines using enriched and optimized protein‐free, cholesterol‐free, and chemically

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In order to develop an efficient process for large-scale production of recombinant protein, various factors were studied which affect the productivity of Sf-9 (Spodoptera frugiperda) insect cells when using the baculovirus expression system. It was shown that upon infection with the Bac-BRV6L recomb