Adult bone and mineral working group WG1–WG9

• Publisher: American Society for Bone and Mineral Research
• Year: 2010
• Tongue: English
• Weight: 213 KB
• Volume: 25
• Category: Article
• ISSN: 0884-0431
• DOI: 10.1002/jbmr.5650251306

✦ Synopsis

ACTH, leaving cells in dexamethasone alone for 36 hours, caused a marked increase in annexin V-positivity, proving that ACTH was critical for cell survival. VEGF production was also induced by co-culturing human osteoblasts with CD14+ osteoclast precursors. Glucocorticoids abolished this effect, showing that endogenous VEGF in bone was sensitive to glucocorticoid inhibition, and was perhaps causal to the osteonecrosis. Finally, a modest stimulation of early osteoclastogenesis was noted in RAW264.7 cells and murine osteoclast precursors, with little or no effects on bone resorption by mature osteoclasts. The results not only substantiate our view regarding a novel pituitary-bone axis, in which stimulating hormones, such as ACTH, FSH and TSH, bypass traditional endocrine targets to effect the skeleton directly, but also, on a broader clinical front, call for studies to examine the efficacy of ACTH in preventing human osteonecrosis Disclosures: Jammel Iqbal, None.