A protocol is described for coupling of carrier-free iodine to protein sulfhydryl groups via fluorescein maleimide. 125I is first coupled to fluorescein maleimide in the presence of chloramine T. Iodination is stopped with sodium thiosulfate, and the iodine-substituted fluorescein maleimide is react
Adsorption of fluorescein by protein crystals
β Scribed by A. Cvetkovic; M. Zomerdijk; A.J.J. Straathof; R. Krishna; L.A.M. van der Wielen
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 299 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0006-3592
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