Adriamycin-induced DNA strand breaks in HeLa and in P388 leukaemia cells detected using in situ nick translation
β Scribed by Yoshihiko Maehara; Yasunori Emi; Hideaki Anai; Yoshihisa Sakaguchi; Shunji Kohnoe; Shunichi Tsujitani; Keizo Sugimachi
- Book ID
- 104506251
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 471 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0022-3417
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This laboratory has recently reported the occurrence of DNA nicking at the onset of terminal skeletal myogenesis by using the technique of in situ nick translation (Dawson and Lough: Dev. Bio/., 1271362-367, 1988). Because 1 -beta-D-arabinofuranosylcytosine (araC), a cytocidal agent that is routinel
The detection of DNA single-strand breaks (SSB) in human mononucleated white blood cells (MWBC) using a modified version of the nick translation assay is presented. This assay allows rapid and sensitive examination of SSB using only 5 ml heparinized blood fo'~ an eightfold determination. The assay w