ADP-Ribosylation of Membrane Proteins in Cholinergic Nerve Terminals
✍ Scribed by Henry A. Lester; Michael L. Steer; Daniel M. Michaelson
- Book ID
- 111171810
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 745 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0022-3042
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## Abstract ADP‐ribosylation reactions in nucleoli of exponentially growing HeLa cells were studied. Isolated nuclei or nucleoli were labeled with ^32^P‐NAD; then the nucleolar proteins were analyzed by 1‐dimensional and 2‐dimensional polyacrylamide gel electrophoresis (PAGE) and modified proteins
Decreases in hepatocyte NAD+ produced by ethanol are only partially explained by the increased conversion of NAD+ to NADH and NADP+. The purpose of this study was to determine whether a mechanism for the ethanol-induced decrease in NAD+ is its increased use in ADP-ribosylation. Exposure of hepatocyt