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ADP-Glo: A Bioluminescent and Homogeneous ADP Monitoring Assay for Kinases

✍ Scribed by Zegzouti, Hicham; Zdanovskaia, Marina; Hsiao, Kevin; Goueli, Said A.


Book ID
121763044
Publisher
Mary Ann Liebert
Year
2009
Tongue
English
Weight
680 KB
Volume
7
Category
Article
ISSN
1540-658X

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A new method for the measurement of poly(ADP-ribose) is described. Poly(ADP-ribose) was separated from the bulk of cellular RNA and DNA by quantitative adsorption to dihydroxyboryl-Sepharose. The polymer was digested with venom phosphodiesterase and bacterial alkaline phosphatase to yield the uniqu