## Abstract Previous work in our laboratory led us to postulate that N2a cells release adenosine into growth medium, where it acts at the extracellular adenosine receptors to modulate the sensitivity of the cells to the cyclic AMPβelevating effect of adenosine [Green, RD, J Pharmacol Exp Ther 201:6
Adenosine transport by a variant of C1300 murine neuroblastoma cells deficient in adenosine kinase
β Scribed by Richard D. Green
- Book ID
- 115724433
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 543 KB
- Volume
- 598
- Category
- Article
- ISSN
- 0005-2736
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DEAE-cellulose chromatography of the 20,000g supernatant fraction of homogenates of C-1300 murine neuroblastoma (clone N2a) yields one major and two minor peaks of cyclic AMP-dependent protein kinase activity. Assessment of the endogenous activation state of the enzyme(s) reveals that the enzyme is
## Abstract Cyclic adenosine 3β²:5β²βmonophosphate (cAMP)βdependent protein kinases from three clones of C1300 neuroblastoma, established as growing tumors in A/jax mice, were identified and characterized. Mean (Β± SD) intratumor concentrations of cAMP ranged from 5.0Β±2.4 to 6.8Β±1.9 pmol/mg protein; c