✦ LIBER ✦

Additional microsatellite markers for mouse genome mapping

✍ Scribed by Catherine M. Hearne; Marcia A. McAleer; Jennifer M. Love; Timothy J. Aitman; Richard J. Cornall; Soumitra Ghosh; Andrew M. Knight; Jan-Bas Prins; John A. Todd

Publisher: Springer-Verlag
Year: 1991
Tongue: English
Weight: 887 KB
Volume: 1
Category: Article
ISSN: 0938-8990
DOI: 10.1007/bf00352339

No coin nor oath required. For personal study only.

✦ Synopsis

Mouse sequence information from the EMBL and GenBank databases, published sequences and genomic clones have been analyzed for simple repetitive elements or microsatellites. Each microsatellite has been amplified by the polymerase chain reaction (PCR) as a single locus marker. PCR primers were designed from unique sequence flanking each repeat. Size variation of PCR products less than 750 base pairs (bp) between mouse strains has been determined using ethidium bromide-stained acrylamide or agarose gels. A further 74 newly characterized microsatellites are presented in this paper, bringing to 185 the total we have analyzed. Of these, 157/185 (85%) have more than one allele, 143/178 (80%) vary in length between C57BL/6J and Mus spretus, and 82/168 (49%) vary between DBA/2J and C57BL/6J. Microsatellites provide informative single locus probes for linkage analysis in the construction of a genetic map of the mouse genome.

📜 SIMILAR VOLUMES

Typing recombinant inbred mouse strains

Typing recombinant inbred mouse strains for microsatellite markers

✍ Benjamin A. Taylor; Peter C. Reifsnyder 📂 Article 📅 1993 🏛 Springer-Verlag 🌐 English ⚖ 404 KB

In total, 41 different microsatellite variants have been typed in one or more of four different sets of recombinant inbred (RI) mouse strains. Microsatellite variants were selected that were located in chromosomal regions previously lacking markers. These markers extend the regions swept in these RI

PCR-analyzed microsatellites of the mous

PCR-analyzed microsatellites of the mouse genome—additional polymorphisms among ten inbred mouse strains

✍ George A. Fowlis; Simon Adelman; Andrew M. Knight; Elizabeth Simpson 📂 Article 📅 1992 🏛 Springer-Verlag 🌐 English ⚖ 643 KB

Eighty sequences from the mouse genome database containing microsatellites (simple sequence repeats) have been analyzed for size variation among ten different inbred strains of mice; 62/80 (77.5%) showed polymorphism of at least three alleles. We have been able to detect all the polymorphisms by aga

A synteny map of the horse genome compri

A synteny map of the horse genome comprised of 240 microsatellite and RAPD markers

✍ Y-L. Shiue; L. A. Bickel; A. R. Caetano; L. 5V. Millon; R. S. Clark; M. L. Eggle 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 89 KB

Development of microsatellite markers an

Development of microsatellite markers and comparative mapping for bovine chromosome 19

✍ B. W. Kirkpatrick; B. Byla; E. Kurar; W. C. Warren 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 120 KB

Previous research has mapped an ovulation rate quantitative trait locus (QTL) to bovine chromosome 19. In an effort to enhance comparative mapping information and develop additional markers for refined QTL mapping, microsatellite markers were developed in a targeted approach. A bovine bacterial arti

Thirty-one polymorphic microsatellite ma

Thirty-one polymorphic microsatellite markers for genetic mapping in rainbow trout (Oncorhynchus mykiss)

✍ A. Ozaki; S.-K. Khoo; T. Sakamoto; N. Okamoto 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 454 KB

Genomics (Essential Methods) || Identifi

Genomics (Essential Methods) || Identification of Polymorphic Markers for Genetic Mapping

✍ Starkey, Mike; Elaswarapu, Ramnath 📂 Article 📅 2010 🏛 John Wiley & Sons, Ltd ⚖ 589 KB

Single nucleotide polymorphisms (SNPs) are the most prevalent form of DNA sequence variation in humans. At the end of 2008, public databases contained more than 12 million entries of genetic variants in humans, the vast majority of which are SNPs [1]. While the incidence of SNPs in the human genome