PCR-analyzed microsatellites of the mouse genome—additional polymorphisms among ten inbred mouse strains

✍ Scribed by George A. Fowlis; Simon Adelman; Andrew M. Knight; Elizabeth Simpson

• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 643 KB
• Volume: 3
• Category: Article
• ISSN: 0938-8990
• DOI: 10.1007/bf00355718

✦ Synopsis

Eighty sequences from the mouse genome database containing microsatellites (simple sequence repeats) have been analyzed for size variation among ten different inbred strains of mice; 62/80 (77.5%) showed polymorphism of at least three alleles. We have been able to detect all the polymorphisms by agarose gel electrophoresis, often running the gels for up to 3 h. Between individual pairs of mouse strains to be used in chromosomal mapping studies in our laboratory, 35-60% polymorphism occurred. There are potentially enough microsatellites within the mouse and human genome to have a marker at every 1-cM distance. This simple approach will, therefore, continue to be useful in genome mapping studies, leading eventually to high-resolution maps of both the mouse and human genomes; this should allow for physical mapping and cloning of specific genes.