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Addendum to ‘a simple effective embryo culture technique forBrassica’

✍ Scribed by D. J. Harberd

Book ID
104641279
Publisher
Springer
Year
1971
Tongue
English
Weight
40 KB
Volume
20
Category
Article
ISSN
0014-2336

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✦ Synopsis

In the earlier paper I did not mention, inadvertently, that "White's" medium as used here has in fact been updated from the original formulation by the substitution in place of ferric sulphate of 10 cm3/litre of a stock iron solution made up as follows: Disodium ethylene diamine tetraacetic acid 0.8 g Ferric chloride 0.1 g Distilled water to I litre.

📜 SIMILAR VOLUMES

A simple effective embryo culture techni
A simple effective embryo culture technique for brassica
✍ D. J. Harberd 📂 Article 📅 1969 🏛 Springer 🌐 English ⚖ 281 KB

Sterilized ovules of a suitable age are cut in half and introduced to a culture medium kept in constant motion. Many of the embryos are washed out of the ovules and grow. The success rate compares well with more sophisticated techniques. Several plants of synthetic B. napus have been grown by this m