In the earlier paper I did not mention, inadvertently, that "White's" medium as used here has in fact been updated from the original formulation by the substitution in place of ferric sulphate of 10 cm3/litre of a stock iron solution made up as follows: Disodium ethylene diamine tetraacetic acid 0.8
β¦ LIBER β¦
A simple effective embryo culture technique for brassica
β Scribed by D. J. Harberd
- Book ID
- 104756434
- Publisher
- Springer
- Year
- 1969
- Tongue
- English
- Weight
- 281 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0014-2336
No coin nor oath required. For personal study only.
β¦ Synopsis
Sterilized ovules of a suitable age are cut in half and introduced to a culture medium kept in constant motion. Many of the embryos are washed out of the ovules and grow. The success rate compares well with more sophisticated techniques. Several plants of synthetic B. napus have been grown by this method.
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