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Adaptation of BHK cells producing a recombinant protein to serum-free media and protein-free medium

✍ Scribed by Helder J. Cruz; José L. Moreira; Glyn Stacey; Elsa M. Dias; Katherine Hayes; Dennis Looby; Bryan Griffiths; Manuel J. T. Carrondo

Book ID
110256654
Publisher
Springer
Year
1998
Tongue
English
Weight
57 KB
Volume
26
Category
Article
ISSN
0920-9069

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Five mouse-human hybridoma cell lines secreting human IgM class monoclonal antibodies were examined for possible adaptation to a protein free medium. Four were successfully adapted to the medium. The adapted cell lines could be cultured for more than two months without any changes in their cell grow