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Activity of 3β-hydroxysteroid dehydrogenase Δ4–5isomerase in the human skin

✍ Scribed by S. Itami; S. Takayasu

Publisher
Springer-Verlag
Year
1982
Tongue
English
Weight
307 KB
Volume
274
Category
Article
ISSN
0340-3696

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✦ Synopsis

The activity of 3 beta-hydroxysteroid dehydrogenase delta 4-5 isomerase (3 beta-HSD) was assayed in various tissues microdissected from the freeze-dried human skin of 13 subjects. The sebaceous gland possessed the highest activity of 3 beta-HSD in the skin, while the apocrine sweat gland showed only one fourth of that activity. The vellus hair follicle showed nearly one half of the activity of the sebaceous gland, whereas the terminal hair follicle exhibited much lower activity. The activity of the epidermis and of the dermis were negligible. Incubation of fresh whole skin of the forehead with 7 alpha-3H-DHA and subsequent isolation of various tissues revealed that the metabolites identified in the sebaceous gland were androstanedione and androstenedione, whereas testosterone and/or dihydrotestosterone were not detected.

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