✦ LIBER ✦
Δ5-3β-hydroxysteroid dehydrogenase/Δ5-Δ4-ketosteroid isomerase (3β-HSD), a possible enzyme of cardiac glycoside biosynthesis, in cell cultures and plants ofDigitalis lanataEHRH
✍ Scribed by Stefanie Seidel; Wolfgang Kreis; Ernst Reinhard
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 380 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0721-7714
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✦ Synopsis
The in vitro transformation of pregnenolone into progesterone in Digitalis lanata tissues was shown to be catalyzed by a 3β-hydroxysteroid dehydrogenase/ketosteroid isomerase (3β-HSD). Product formation was monitored by HPLC. The enzyme could be partially characterized and 3β-HSD activities were measured in various Digitalis lanata tissues and in cell cultures of other plant species. Since no correlation was observed between biosynthetic competence of the tissue and 3β-HSD activity, it was concluded that this enzyme does not play a major role in regulating cardenolide biosynthesis.