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Activity losses among T4 lysozyme variants after adsorption to colloidal silica

โœ Scribed by C. K. Bower; Q. Xu; J. McGuire

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
104 KB
Volume
58
Category
Article
ISSN
0006-3592

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โœฆ Synopsis

Maintaining a specific molecular conformation is essential for the proper functioning of an enzyme. A substantial loss of catalytic activity can occur from the displacement caused by even a single amino acid substitution. Activity may also be lost as an enzyme undergoes a conformational change during adsorption. In this study, we investigated the effect of thermostability on the activities of three T4 lysozyme variants after adsorption to 9 nm colloidal silica particles. Less-stable T4 lysozyme variants lost more activity after adsorption than did more stable variants, apparently because they experienced more extensive structural alteration.

๐Ÿ“œ SIMILAR VOLUMES

Activity losses among T4 lysozyme charge
Activity losses among T4 lysozyme charge variants after adsorption to colloidal silica
โœ C. K. Bower; S. Sananikone; M. K. Bothwell; J. McGuire ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 80 KB

Enzyme structure and function depend to some extent on enzyme net charge and charge location. Altering the charge of even a single residue may affect the interaction between enzyme and substrate such that all catalytic activity is lost. In this study we investigated the effect of net charge and char