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Activity-Based Fingerprinting of Proteases

✍ Scribed by Rajavel Srinivasan; Xuan Huang; Su Ling Ng; Shao Q. Yao

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
293 KB
Volume
7
Category
Article
ISSN
1439-4227

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## Abstract We have successfully developed a protease assay using fluorescence resonance energy transfer based peptide libraries, which allows not only general detection of enzymatic activities, but more importantly substrate fingerprinting of proteases from different classes. The method allows the