Chloride uptake by the cyanobacterium Anacystis nidulans at 38 ~ C is energy dependent showing maximum rate (around 5.10 -7 tool C1-โข ml cell water -1 โข rain -1) and accumulation (up to 160 fold) in light and air. The respective values in air and darkness were 40-70 ~ lower. In the dark under N~ no uptake was found. Chloride transport had an optimum at pH 6.7 and a KM of 2.10 -5 M which was pHindependent. It was inhibited by carbonyl cyanide m-chlorophenylhydrazone and N,N'-dicyclohexylcarbodiimide in the light and in the dark, and also to a lesser extent by valinomycin. 3-(3,4-dichlorophenyl)-l,l-dimethylurea in the light caused a moderate stimulation.
To obtain information about the energy source of active chloride transport the action of the four inhibitors on membrane potential (determined through the distribution of triphenylmethylphosphonium) and ATP level (determined by the firefly method) was examined. It was found that a high negative membrane potential was unfavorable for chloride accumulation probably by stimulating passive efflux. On the other hand a good correlation between ATP level and chloride transport activity was obtained.
Attempts to induce chloride uptake by sudden acidification of the external medium in presence of N,N'dicyclohexyl-carbodiimide or during anaerobiosis were not successful.
Two mechanisms of chloride uptake are discussed: a) primary active transport by an ATP-dependent pump, and b) "chemiosmotic" secondary active transport linked to a proton gradient, the present data favoring mechanism a.