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Active-Site Motions and Polarity Enhance Catalytic Turnover of Hydrated Subtilisin Dissolved in Organic Solvents

โœ Scribed by Hudson, Elton P.; Eppler, Ross K.; Beaudoin, Julianne M.; Dordick, Jonathan S.; Reimer, Jeffrey A.; Clark, Douglas S.


Book ID
126892862
Publisher
American Chemical Society
Year
2009
Tongue
English
Weight
357 KB
Volume
131
Category
Article
ISSN
0002-7863

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Correlation between catalytic activity a
โœ Kui Xu; Kai Griebenow; Alexander M. Klibanov ๐Ÿ“‚ Article ๐Ÿ“… 1997 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 73 KB ๐Ÿ‘ 2 views

Fourier-transform infrared (FTIR) spectroscopy has been used to quantify the alpha-helix and beta-sheet contents of subtilisin Carlsberg dissolved in several nonaqueous, as well as aqueous, solvents. Independently, the catalytic activity of the enzyme has been measured in the same solvents. While ou