✦ LIBER ✦

Correlation between catalytic activity and secondary structure of subtilisin dissolved in organic solvents

✍ Scribed by Kui Xu; Kai Griebenow; Alexander M. Klibanov

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 73 KB
Volume: 56
Category: Article
ISSN: 0006-3592
DOI: 10.1002/(sici)1097-0290(19971205)56:5<485::aid-bit2>3.0.co;2-e

No coin nor oath required. For personal study only.

✦ Synopsis

Fourier-transform infrared (FTIR) spectroscopy has been used to quantify the alpha-helix and beta-sheet contents of subtilisin Carlsberg dissolved in several nonaqueous, as well as aqueous, solvents. Independently, the catalytic activity of the enzyme has been measured in the same solvents. While our previous FTIR studies revealed no connection between the secondary structure and enzymatic activity for subtilisin suspended in various organic solvents, a very different situation is observed herein for the dissolved enzyme. Specifically, if either the alpha-helix or beta-sheet content in a given solvent is higher or lower than in water, no appreciable enzymatic catalysis is observed. Conversely, when the secondary structure of subtilisin dissolved in a given nonaqueous solvent is similar to that in water, so is the enzymatic activity. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 485-491, 1997.

📜 SIMILAR VOLUMES

Catalytic activity and conformation of c

Catalytic activity and conformation of chemically modified subtilisin Carlsberg in organic media

✍ Oh Hyeong Kwon; Yukio Imanishi; Yoshihiro Ito 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 55 KB 👁 2 views

Subtilisin Carlsberg, an alkaline protease from Bacillus licheniformis, was modified with polyoxyethylene (PEG) or aerosol-OT (AOT), and the solubility, conformation, and catalytic activity of the modified subtilisins in some organic media were compared under the same conditions. The solubility of m

Can conformational changes be responsibl

Can conformational changes be responsible for solvent and excipient effects on the catalytic behavior of subtilisin Carlsberg in organic solvents?

✍ Kai Griebenow; Alexander M. Klibanov 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 117 KB 👁 2 views

We developed an FTIR (Fourier transform infrared) methodology for quantitatively assessing the secondary structure of proteins suspended in nonaqueous media. This methodology was used to measure the percentages of ␣-helices and ␤-sheets of subtilisin Carlsberg, prepared under different conditions, p

Structure of lysozyme dissolved in neat

Structure of lysozyme dissolved in neat organic solvents as assessed by NMR and CD spectroscopies

✍ Tatyana Knubovets; John J. Osterhout; Alexander M. Klibanov 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 121 KB 👁 1 views

The structure of the model protein hen eggwhite lysozyme dissolved in water and in five neat organic solvents (ethylene glycol, methanol, dimethylsulfoxide (DMSO), formamide, and dimethylformamide (DMF)) has been examined by means of 1 H NMR and circular dichroism (CD) spectroscopies. The NMR spectr

Synthesis of Optically Active α-Amino- p

Synthesis of Optically Active α-Amino- phosphinic Acids by Catalytic Asymmetric Hydrogenation in Organic Solvents and Aqueous Micellar Media

✍ Torsten Dwars; Ute Schmidt; Christine Fischer; Ingrid Grassert; Rhett Kempe; Rol 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 106 KB 👁 1 views

Activation of a Mo-V-W-O Catalyst — Corr

Activation of a Mo-V-W-O Catalyst — Correlation of Catalytic Performance in the Acrolein Oxidation and Structural Changes of the Catalyst

✍ O. Ovsitser; A. Blume; Y. Uchida; G. Mestl; R. Schlögl 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 German ⚖ 42 KB 👁 1 views

Preparation of copoly(vinyl alcohol–styr

Preparation of copoly(vinyl alcohol–styrenesulfonic acid) resin and its catalytic activity on hydrolysis of carbohydrates. IV. Hydrolysis of dextrin in mixtures of organic solvents with water

✍ Kenichiro Arai; Yoshitaka Ogiwara; Chiharu Kuwabara 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 258 KB