Active site-directed and allosteric effectors of regulatory enzymes: The activation of aspartate transcarbamylase by substrate and transition state analogues

The Structures of the Enzyme–Substrate C

The Structures of the Enzyme–Substrate Complex and Transition State Formed in the SN2 Displacement of Cl−from 1,2-Dichloroethane at the Active Site ofXanthobacter autotrophicusHaloalkane Dehalogenase

✍ Felice C. Lightstone; Ya-Jun Zheng; Thomas C. Bruice 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 208 KB

The mechanism of an enzymatic reaction is better understood when the structures of ground and transition states are known. We have set out to determine enzymesubstrate (ES) and transition state (TS) for the S N 2 displacement of Cl Ϫ from 1,2dichlorethane (DCE) by Asp 124-CO Ϫ 2 at the active site o