Activation of Unfertilized Sea Urchin Eggs by Trypsin
β Scribed by JENSEN, AAGE BOHUS
- Book ID
- 109558599
- Publisher
- Nature Publishing Group
- Year
- 1948
- Tongue
- English
- Weight
- 231 KB
- Volume
- 162
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/162931a0
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## Abstract After fertilization the sea urchin sperm nucleus transforms into the male pronucleus which later fuses with the female pronucleus reβestablishing the diploid genome of the embryo. This process requires remodeling of the sperm chromatin structure including the replacement of the sperm hi
release was induced by intracoelomically treating the specimens with 0.5M KC1. The harvested eggs were then rinsed thoroughly in Instant Ocean, (Aquarium Systems Co., Mentor, Ohio) (pH 7.801, a synthetic marine water mix. Male urchin testes were removed, placed in a petri dish, and maintained at 4Β°