✦ LIBER ✦

Activation of the K-ras oncogene in colorectal neoplasms is Associated with decreased apoptosis

✍ Scribed by Robyn L. Ward; Alison V. Todd; Fernando Santiago; Terence O'Connor; Nicholas J. Hawkins

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 168 KB
Volume: 79
Category: Article
ISSN: 0008-543X
DOI: 10.1002/(sici)1097-0142(19970315)79:6<1106::aid-cncr8>3.0.co;2-d

No coin nor oath required. For personal study only.

✦ Synopsis

METHODS.

Mutations in codon 12 of K-ras were determined by a single tube, enriched polymerase chain reaction. Apoptotic cells in tumor sections were identi-1 Department of Medical Oncology, St. Vincent's fied by in situ end-labeling of fragmented DNA, whereas levels of bcl-2 and p53 Hospital, Darlinghurst, Australia.

proteins were determined by immunohistochemistry. 2 R. W. Jones Pharmaceutical Research Insti-

RESULTS.

Tumors with mutant K-ras had a significantly lower apoptotic index than tute, Sydney, Australia.

those with the wild-type allele (P õ 0.05). They were also more likely to exhibit positive bcl-2 staining (P õ 0.05). Adenomas showed significantly greater bcl-2

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