The nuclear run-on transcription assay is the only approach to measure the transcriptional activity of a given gene in its genuine structural and regulatory cellular context. However, serious problems in the interpretation of results can arise from the artificial activation of paused RNA polymerases
Activation of rotavirus RNA polymerase by calcium chelation
β Scribed by J. Cohen; J. Laporte; A. Charpilienne; R. Scherrer
- Publisher
- Springer Vienna
- Year
- 1979
- Tongue
- English
- Weight
- 608 KB
- Volume
- 60
- Category
- Article
- ISSN
- 1432-8798
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## Abstract The carboxylβterminal domain (CTD) of the largest subunit of mammalian RNA polymerase II (RNAP II) consists of 52 repeats of a consensus heptapeptide and is subject to phosphorylation and dephosphorylation events during each round of transcription. RNAP II activity is regulated during t