Activation of Ras, Raf-1 and protein kinase C in differentiating human neuroblastoma cells after treatment with phorbolester and NGF
✍ Scribed by Helena Söderholm; Anna-Karin Olsson; Erik Lavenius; Lars Rönnstrand; Eewa Nånberg
- Book ID
- 114283529
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 452 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0898-6568
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The role of protein kinase C activation in changes in muscarinic receptor functions and in the appearance of biochemical properties characteristic of neuronal cells was studied in SH-SY5Y human neuroblastoma cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (
The effects of phorbol esters on Ca 2 + channel currents in human neuroblastoma SH-SY5Y cells were studied using whole-cell patch-clamp recordings. Bath application of 12-O-tetradecanoylphorbol-13-acetate (TPA) or phorbol 12, 13-dibutyrate (PDBu; 100 nM to 1 #M), known activators of protein kinase C