Activation of natural killer cells via the FcγRIII (CD16) requires initial tyrosine phosphorylation

✍ Scribed by Anders Ståhls; Marja Heiskala; Tomas Mustelin; Leif C. Andersson

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 419 KB
• Volume: 22
• Category: Article
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830220249

✦ Synopsis

Activation of natural killer cells via the FcyRIII (CD16) requires initial tyrosine phosphorylation*

Triggering of the FcyRIII (CD16) on natural killer (NK) cells by monoclonal antibodies or antibody-coated target cells stimulates a rapid phospholipase C (PLC)-mediated hydrolysis of inositol phospholipids and results in subsequent delivery of the lytic hit.The role of initial tyrosine phosphorylation in these events was investigated with a tyrosine protein kinase (TPK) inhibitor, genistein. At doses that inhibited CD16-triggered tyrosine phosphorylation of substrates in intact cells, genistein did not influence serinehhreonine phosphorylation or target cell binding but prevented PLC activation, cell-mediated cytotoxicity and antibody-dependent cellular cytotoxicity. These findings indicate that tyrosine phosphorylation is an early and critical event during receptor-mediated activation of the lytic machinery.