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Activation of MAP kinase and enhanced phosphorylation of the 350-kDa protein by mitogenic stimuli in quiescent Balb/c 3T3 cells

โœ Scribed by Minoru Kawakami; Eisuke Nishida; Kazuyuki Tobe; Minako Hoshi; Takashi Kadowaki; Masato Kasuga; Hikoichi Sakai


Book ID
115892652
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
878 KB
Volume
193
Category
Article
ISSN
0014-4827

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Platelet-derived growth factor (PDGF) and other agents that activate protein kinase C (PKC) rapidly alter cytosolic pH (pHJ and intracellular free calcium (Ca' +I,) in BALBk-3T3 fibroblasts. To define whether changes in pH, or [Ca++], are linked to PDGF-stimulated mitogenesis, these parameters were