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Activation of beef heart mitochondrial adenosine triphosphatase by 2,4-dinitrophenol

✍ Scribed by Cantley, Lewis C.; Hammes, Gordon G.

Book ID: 127116190
Publisher: American Chemical Society
Year: 1973
Tongue: English
Weight: 621 KB
Volume: 12
Category: Article
ISSN: 0006-2960
DOI: 10.1021/bi00748a014

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## Abstract Tightly bound adenine nucleotides are removed from multiple binding sites on beef heart mitochondrial ATPase (F~1~) by chromatography on columns of Sephadex equilibrated with 50% glycerol. Release of nucleotides from the enzyme is associated with large decreases in sedimentation velocit