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The crystallization of beef heart mitochondrial adenosine triphosphatase

โœ Scribed by Vitaly Spitsberg; Robert Haworth


Book ID
113125555
Publisher
Elsevier Science
Year
1977
Weight
584 KB
Volume
492
Category
Article
ISSN
0005-2795

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Physical and enzymatic properties of nuc
โœ Garrett, Neil E. ;Penefsky, Harvey S. ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 535 KB

## Abstract Tightly bound adenine nucleotides are removed from multiple binding sites on beef heart mitochondrial ATPase (F~1~) by chromatography on columns of Sephadex equilibrated with 50% glycerol. Release of nucleotides from the enzyme is associated with large decreases in sedimentation velocit