✦ LIBER ✦

Action of tolytoxin on cell morphology, cytoskeletal organization, and actin polymerization

✍ Scribed by Patterson, Gregory M. L. ;Smith, Charles D. ;Kimura, Lucille H. ;Britton, Bruce A. ;Carmeli, Shmuel

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 851 KB
Volume: 24
Category: Article
ISSN: 0886-1544
DOI: 10.1002/cm.970240105

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✦ Synopsis

Tolytoxin, a cytostatic, antifungal macrolide produced by blue-green algae of the genus Scytonema, is a potent, reversible inhibitor of cytokinesis in cultured mammalian cells. Treatment of KB cells with 2-16 nM tolytoxin results in profound morphological changes, beginning with the formation of zeiotic processes and culminating in nuclear protrusion. In L1210 cells, cytokinesis is inhibited by as little as 2 nM tolytoxin, while karyokinesis proceeds normally, resulting in polynucleation. Tolytoxin specifically disrupts microfilament organization in A 10 cells, while having no apparent effect on microtubules or intermediate filaments.

Tolytoxin inhibited actin polymerization in vitro and also caused the depolymerization or fragmentation of F-actin in vitro. Tolytoxin exhibits effects that closely resemble those of cytochalasin B but is effective at concentrations 1/50-l/l,OOO that of cytochalasin B.

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