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ACSD labelling and magnetic cell separation: a rapid method of separating antibody secreting cells from non-secreting cells

✍ Scribed by Silvia Carroll; Mohamed Al-Rubeai


Book ID
113716907
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
595 KB
Volume
296
Category
Article
ISSN
0022-1759

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A method is described for the separation and assay of radiolabeled glycosaminoglycuronans (GAGS) from cell culture medium. Following papain digestion and precipitation with cetylpyridinium chloride onto cellulose ester filter membrane, hyaluronic acid can be separated from sulfated GAGS by selective