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Acridine orange staining of the mammalian fibroblast cell coat

✍ Scribed by J. Timár; G. Gyapay; K. Lapis

Publisher
Springer
Year
1979
Tongue
English
Weight
739 KB
Volume
64
Category
Article
ISSN
1432-119X

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✦ Synopsis

Acridine orange selectively binds to glycos- and galactosaminoglycan (GAG) compounds in the presence of Na+ in low concentrations. We have worked out a cytochemical method, which is suitable for the specific demonstration of surface GAG components. The method consists of a glutaraldehyde prefixation, an acridine orange block-staining for 48 h and an OsO4 postfixation for some hours. The specificity of the staining was verified with the help of fluorimetric--in vitro--measurements and enzymatic digestions, respectively.

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